Fig. 2: S4 mediated GTP hydrolysis and nucleotide exchange.
From: Structure and function of a near fully-activated intermediate GPCR-Gαβγ complex
a GTP hydrolysis of the S4 mediated Gαsβγ as a function of inverse, partial, and full agonists, in reference to Gαsβγ alone (negative control) and the S5 mediated Gαsβγ (positive control). Data with error bars are presented as mean ± SEM of four independent experiments. Statistical analyses were performed using the ordinary one-way ANOVA compared to the R291A apo, ***p < 0.001, and ****p < 0.0001. b Time course of GTP hydrolysis of the S4 mediated Gαsβγ, in reference to Gαsβγ alone (negative control) and the S5 mediated Gαsβγ (positive control); the initial rate of each catalysis was calculated. The initial rate of each catalysis was calculated. Data are presented as mean values ± SD from three independent experiments (n = 3). Source data are provided in the Source Data file. c Km values for S4 mediated Gαsβγ, in reference to Gαsβγ alone (negative control) and the S5 mediated Gαsβγ (positive control). Data are presented as mean values ± SD from three independent experiments (n = 3). Source data are provided in the Source Data file. d BODIPY-FL-GDP binding assay. e BODIPY-FL-GTP binding assay. f BODIPY-FL-GTP-γ-S binding assay (g) GTP-GDP exchange rate comparison. h The structures of BODIPY-FL-GDP, BODIPY-FL-GTP, and BODIPY-FL-GTP-γ-S.
