Fig. 3: Photochemical characterization of cPlk1i.

a Absorption and emission spectra of cPlk1i. b Uncaging reaction of cPlk1i releasing the active Plk1 inhibitor Plk1i. c Uncaging of cPlk1i monitored by ¹H NMR. The NMR sample (CH₃CN:D₂O) was illuminated with 455 nm light at 8.3 mW/cm² for the indicated times. The reference spectrum of Plk1i without the coumarin cage is below in red. Orange boxes: signals of protons a and b used to quantify uncaging, shown in (d). The assigned spectra are shown in Supplementary Fig. 2a and b. e Uncaging of cPlk1i monitored by LC-MS. Absorption chromatogram at 260 nm (top) and 450 nm (bottom). Source data are provided as a Source Data file.