Fig. 7: Schematic summary of in cellulo substitution assays. | Nature Communications

Fig. 7: Schematic summary of in cellulo substitution assays.

From: Kingdom-specific lipid unsaturation calibrates sequence evolution in membrane arm subunits of eukaryotic respiratory complexes

Fig. 7

Top left: Human Ndufa1-EGFP (Hs-A1; blue) stably integrate into CI in HEK293T cells. Bottom left: Chimera Ndufa1-EGFP (Ch-A1) – Hs-A1 trans-IMM helix exchanged with At-A1, Hs-A1FP/LL – Hs-A1 with lipid exposed amino acids F3 and P7 exchanged with At-A1 amino acid L3 and L7, Hs-A1Ex-switch – Hs-A1 with lipid exposed amino acids exchanged with At-A1 could not stably integrate into CI holocomplex and supercomplexes. Top right: treating cells with 18:2 fatty acid (Linoleic acid) does not perturb CI. Bottom right: treating cells with 18:3 fatty acid (alpha-linolenic acid) destabilizes CI.

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