Fig. 8: The establishment of long-term, three-dimensional cultures of human cervical precancerous organoids and their utility.
From: Microbial metabolites control self-renewal and precancerous progression of human cervical stem cells
a Time course images of human cervical precancerous HSIL organoids over 16 days. b Organoid-forming efficiency of HSIL organoids in our cervical organoid culture medium (n = 4). c Somatic mutations called from the cervical HSIL lesions. Mutation profiles are derived from organoids from primary HSIL cells or organoids within passage number 2. d H&E staining of human HSIL lesion. e H&E staining of human HSIL organoids. Asterisk: anisocytosis. Arrowhead: intercellular bridge. Arrow: keratin pearl. f Immunohistochemistry staining of human HSIL lesion. g Immunohistochemistry staining of human HSIL organoids. h, i The effect of L. iners CFS on human normal ectocervical organoid formation. Representative bright-field images (h) and bar graph for relative numbers (i) of organoids (n = 3). j, k, The effect of L. iners CFS on human HSIL organoid formation. Representative bright-field images (j) and bar graph for relative numbers (k) (n = 4). The effect of lactate isomers on normal cervical organoid formation. Representative bright-field images (l) and bar graph for relative numbers (m) (n = 4). The effect of lactate isomers on HSIL organoid formation. Representative bright-field images (n) and bar graph for relative numbers (o) (n = 4). p The effect of lactate isomers on cell proliferation of HeLa cervical cancer cell line (n = 3). q, r, The effect of LY294002 on HSIL organoid formation. Representative bright-field images (q) and bar graph for relative numbers (r) (n = 3). s Model of Lactobacilli metabolite in human cervical stem cells. Lactobacilli-derived lactate regulates normal and precancerous stem cells in the human cervix and interferes with HPV-induced tumorigenesis. Created in BioRender. Myeong, J. (2025) https://BioRender.com/a50d432. All data are collected from indicated biological replicates and presented as mean ± SEM (*p < 0.05; **p < 0.01; ***p < 0.001). P-values were calculated by two-tailed unpaired Student’s t-test (I, k) or one-way ANOVA with Tukey’s multiple comparison test (m, o, p, r). Source data and exact p-values are provided as a Source Data file. All scale bars: 100 μm.
