Fig. 7: AHL10-mediated transcriptional repression of DREB2A, ANAC040 and MYB15.

a ChIP-seq analysis identifies AHL10-enriched peaks. b Distribution of SlVOZ1 binding peaks across the Arabidopsis genome. c The five enriched binding motifs identified for AHL10. d Venn diagram identifies target genes co-regulated by AHL10-CDK8. e Schematic diagram of the MARs and potential AHL10 binding sites in DREB2A, ANAC040 and MYB15 promoters. Red lines indicate MARs; blue boxes indicate exons; red arrows indicate the ___location of AT-rich motifs on MARs. f EMSA showing the binding of AHL10 to the FAM-labeled AT-rich probe. g Schematic diagrams of the effector and reporter constructs used for the transactivation assay. h LUC/REN ratio showing CDK8 and AHL10-mediated transcriptional repression of DREB2A, ANAC040, and MYB15. Values are means ± SD (n = 3). Different letters indicate statistically significant differences by one-way ANOVA (Tukey’s multiple comparisons test, P < 0.01). i, j ChIP-qPCR showing the enrichment of AHL10 at the MARs of DREB2A, MYB15 and ANAC040 promoters. WT, AHL10-GFP/WT and AHL10-GFP/cdk8 seedlings were treated with and without NaCl. ChIP–qPCR data were normalized to internal inputs. ACTIN7 was used as a control. Enrichment on the promoters of the indicated genes in WT was set to 1. Values are means ± SD (n = 3). Different letters indicate statistically significant differences by two-way ANOVA (Tukey’s multiple comparisons test, P < 0.01).