Fig. 8: SUVH2/9 negatively regulates plant salt stress responses. | Nature Communications

Fig. 8: SUVH2/9 negatively regulates plant salt stress responses.

From: Salt stress activates the CDK8-AHL10-SUVH2/9 module to dynamically regulate salt tolerance in Arabidopsis

Fig. 8

a Interaction between AHL10 and SUVH2/9 validated by BiFC assays. Scale bar = 50 µm. b Co-IP assays indicating that salt stress reduces the interactions between AHL10 and SUVH2/9 in tobacco. c The root growth of the WT, ahl10 and suvh2/9 seedlings on ½ MS plates with or without 0.1 M NaCl (n = 15). d Relative primary root length ratio of the WT, ahl10, and suvh2/9 seedlings in (c). Values are means ± SD (n = 15). Different letters indicate statistically significant differences by one-way ANOVA (Tukey’s multiple comparisons test, P < 0.01). e ChIP-qPCR showing the enrichment of SUVH2 at the MARs of DREB2A, MYB15 and ANAC040 promoters. f ChIP-qPCR revealing that the enrichment of SUVH2 in salt stress-responsive genes is dependent on AHL10. g ChIP-qPCR showing the fold enrichment of H3K9me2 at the MARs of DREB2A, MYB15 and ANAC040 promoters. WT, cdk8-1, ahl10 and suvh2/9 seedlings were treated with and without NaCl. ChIP–qPCR data were normalized to internal inputs. ACTIN7 was used as a control. Enrichment on the promoters of the indicated genes in WT was set to 1. Values are means ± SD (n = 3). Different letters indicate statistically significant differences by two-way ANOVA (Tukey’s multiple comparisons test, P < 0.01).

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