Fig. 2: Effects of FoxO3 knockout on heart growth in postanal mice.

a Heart weight to tibial length (HW/TL) ratio in control and CKO mice at p1 and p14, respectively (n = 9 mice for p1 and 10 mice for p14). b Representative whole images of control and CKO postnatal hearts at p1 and p14. c Representative H&E staining images of control and CKO postnatal hearts at p1 and p14. d Representative WGA staining images (upper panel) and quantification (lower panel) of cardiomyocyte size in ventricles at p14 (total ~500 cells in 6 hearts per group). e Representative images of cardiomyocytes completely dissociated from whole heart (left panel) and quantification of total rod-shaped cardiomyocyte numbers (right panel) in control and CKO mice at p14 (n = 6 hearts). f Representative images of cardiomyocyte nucleation (left panel) and quantification of mononuclear (Mono), binuclear (Bi), and multinuclear (Multi) cardiomyocytes (right panel) in control and CKO hearts at p14 (n = 6 hearts per group). g, h Representative confocal images (left) and quantification (right) of EdU⁺ (g) and pH3⁺ (h) cardiomyocytes in control and CKO apical ventricles at p1 and p14 (n = 10 hearts). i Representative confocal images at low (left) and high (right) magnification of PCNA⁺ cardiomyocytes in CKO apical ventricles. j Quantification of PCNA⁺ cardiomyocytes in control and CKO apical ventricles at p7 (n = 10 hearts). k, l Representative confocal images (left) and quantification (right) of symmetrical (k) and asymmetrical (l) AurkB⁺ cardiomyocytes in control and CKO apical ventricles at p7 (n = 3 hearts). All data are presented as the mean ± SEM. P values are from two-tailed t test (a, d, e, j–l) or two-way ANOVA followed by Sidak’s multiple comparisons test (f–h). ns, no significant difference. Source data are provided as a Source data file.