Fig. 3: FoxO3 knockout promotes cardiac function in adult mice.

a HW/TL ratio in control and CKO mice at postnatal week 6 (n = 8 mice). b Representative images of H&E staining of sections from control and CKO hearts at postnatal week 6. c The qPCR validation of hypertrophic markers including Anp, Bnp, and Myh7 in control and CKO ventricles at postnatal week 6 (n = 3 hearts). d Representative WGA staining images (left) and quantification (right) of the size of cardiomyocytes located in ventricles at postnatal week 6 (total ~700 cells in 6 hearts per group). e Representative cross section of cTnT staining cardiomyocytes (left) and the quantification of cardiomyocyte size and density (right) in ventricles at postnatal week 6 (n = 6 hearts for control and 10 hearts for CKO). f Representative images of M-model echocardiography (left) and quantification (right) of LVEF and LVFS levels at postnatal week 6 (n = 10 hearts). g HW/TL ratio in control and CKO mice at postnatal week 12 (n = 10 mice). h Representative whole images of adult hearts at postnatal week 12. i Representative images of cardiomyocytes completely dissociated from whole heart in control and CKO mice at postnatal week 12. j Quantification of total cardiomyocytes in control and CKO mice from 2 to 12 weeks (n = 5 hearts). k Representative images of cardiomyocyte nucleation (left) and quantification of mononuclear (Mono), binuclear (Bi), and multinuclear (Multi) cardiomyocytes (right) in control and CKO hearts at postnatal week 12 (n = 3 hearts). l Representative images of M-model echocardiography (left) and quantification of LVEF and LVFS levels (right) at postnatal week 12 (n = 10 hearts). All data are presented as the mean ± SEM. P values are from two-tailed t test (a, d–g, l) or two-way ANOVA followed by Sidak’s multiple comparisons test (c, j, k). ns, no significant difference. Source data are provided as a Source data file.