Fig. 8: FoxO3 knockout enhances the activation of Wnt/β-catenin pathway by suppressing Sfrp2 in cardiomyocytes.

a, b Representative images (a) and quantification of western blotting for Sfrp2 (b, left) and β-catenin (b, right) expression in primary cardiomyocytes isolated from neonatal mice at p1 (n = 3 mice). c Representative images (left) and quantification (right) of immunofluorescent staining for Sfrp2 in control and FoxO3-deficient HL-1 cells (n = ~600 cells from 8 experiments). d Representative images (left) and quantification (right) of immunofluorescent staining for β-catenin in control and FoxO3-deficient HL-1 cells (n = 8). e The relative activation of β-catenin is evaluated in control and FoxO3-deficient HL-1 cells using TOP-Flash reporter assay (n = 5). f, g Representative images (f) and quantification of EdU⁺ HL-1 cells (g, left) and density (g, right) in control and Sfrp2-overexpressing cells (n = 10). h Cell counting assay for control and Sfrp2-overexpressing HL-1 cells (n = 5). i Representative images (left) and quantification (right) of immunofluorescent staining for β-catenin in control and Sfrp2-overexpressing HL-1 cells (n = 6). j The relative activation of β-catenin is evaluated in control and Sfrp2-overexpessing HL-1 cells using TOP-Flash reporter assay (n = 5). All data are presented as the mean ± SEM. P values are from two-tailed t test (b–e, g–j). Source data are provided as a Source data file.