Fig. 1: CoREST complex recruitment requires the full-length KBTBD4R313PRR dimer. | Nature Communications

Fig. 1: CoREST complex recruitment requires the full-length KBTBD4R313PRR dimer.

From: Structural mimicry of UM171 and neomorphic cancer mutants co-opts E3 ligase KBTBD4 for HDAC1/2 recruitment

Fig. 1

a Representative immunoblots of lysates and anti-FLAG immunoprecipitates from HEK293 cells expressing FLAG-KBTBD4R313PRR full-length or Kelch ___domain protein. Immunoprecipitation was performed twice with similar results. Source data in this figure are provided in the Source Data file. b Size-exclusion chromatography showing oligomerization states of KBTBD4R313PRR and its monomeric variant (mono KBTBD4). c Representative immunoblots of lysates and anti-FLAG immunoprecipitates from HEK293 cells expressing FLAG-KBTBD4 variants as indicated. Immunoprecipitation was performed twice with similar results. Source data in this figure are provided in the Source Data file.

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