Fig. 3: PmBF Labeling Preserves OMVs Integrity and Biological Activity. | Nature Communications

Fig. 3: PmBF Labeling Preserves OMVs Integrity and Biological Activity.

From: Specific labeling of outer membrane vesicles with antibiotic-conjugated probe reveals early bacterial infections in blood

Fig. 3

a Confocal imaging of cellular internalization of natural OMVs and PmBF-OMVs, along with quantitative analysis of fluorescence intensity, blue: nucleus; red: OMVs (Bars represent the mean ± SD, n = 3 biological replicates). b Confocal imaging of the colocalization of natural OMVs and PmBF-OMVs with different cellular organelles, accompanied by colocalization coefficients (Lyso: lysosomes, green; Mito: mitochondria, yellow; ER: endoplasmic reticulum, cyan; OMVs: red) (Bars represent the mean ± SD, n = 3 biological replicates). c Scratch experiment of BEAS-2B cells incubated with gradient concentration OMVs or labeled OMVs for 12 or 24 h, control group treated by PBS (Bars represent the mean ± SD, n = 3 biological replicates). a–c were determined by a two-tailed unpaired t-test (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns: P ≥ 0.05). Source data are provided as a Source Data file.

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