Fig. 6: Infiltration of neutrophil resulted in poor prognosis in ATC. | Nature Communications

Fig. 6: Infiltration of neutrophil resulted in poor prognosis in ATC.

From: Integrative proteogenomic characterization reveals therapeutic targets in poorly differentiated and anaplastic thyroid cancers

Fig. 6

A Bar plot showing significantly altered (q value < 0.1) chromosomal arms based on copy number alterations (CNA) in ATC and PDTC samples. B Heatmap showing the correlation between copy number alterations and protein abundance in ATC and PDTC samples. C Scatter plot showing the Spearman correlation between CNA of genes in the 1q chromosomal arm region and their protein abundance. D Venn diagram showing the overlap between cis-effect gene in the 1q region and significantly upregulated protein in ATC. E Expression and survival risk scores of top three proteins from the overlapping section. Heatmap showing protein expression in 20 NT and 113 ATC samples. Whiskers represent 95% confidence interval. F Immunofluorescence staining showing representative micrographs of MPO and citrullinated histone H3 (cit-H3) in normal tissues (NT), PTC, and ATC. n = 3 samples for each group. G Scatter plot showing the Spearman correlation (two-sided) between the protein abundance of NCF2 and neutrophil score calculated based on xCell (n = 159 samples). H, I Spearman correlation (two-sided) of NCF2, MPO, and PAD4 abundance in ATC/PDTC samples (n = 159). J, K Immunofluorescence staining of C5AR1 in NT (n = 30), PTC (n = 35), and ATC (n = 19). ATCs comprised of 9 ATCs that included in the proteomic analysis and belonged to 3 proteomic subtypes (3 samples for each subtype), and 10 ATCs that were not included in the proteomic analysis. Data are presented as mean ± SD, and statistical analysis is performed via one-way ANOVA. L The relevance of C5AR1 and overall survival (Log-rank test) in patients with ATC or PDTC. M, N Spearman correlation (two-sided) of C5AR1, MPO, and PAD4 abundance in ATC/PDTC samples (n = 159). O Heatmap illustrates the expression and activity of STAT1 and regulatory effect on its downstream substrate proteins correlated with neutrophil infiltration. P Flowchart illustrating the potential biological mechanisms associated with poor prognosis in ATC patients. Source data are provided as a Source Data file.

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