Fig. 4: Hemocytes are recruited by cell juxtapositions.
From: Epithelial cell competition is promoted by signaling from immune cells
Flip-out mosaic clones in wing discs at 14 h ACI. Wild-type (A) and dMyc-overexpressing (B, C) clones were created by 10-min (A, B) or 1-hr (C) heat shock. Hemocytes were visualized by srpHemo-H2A-3xmCherry (III) (A–C). Wing discs carrying control clones (D), dMyc clones (E) and a nearly uniform field of cells expressing two copies of dMyc (F), 72 h ACI; hemocytes visualized using hml-Gal4 to drive UAS-RFP. (D’–F’) Wing discs in (D–F) were stained with DAPI. Numbers of hemocytes in wing discs of the genotypes in A–C (G) or D–F (H). *P < 0.05; **P < 0.01, by two-sided Mann-Whitney U test. P value = 8.14 × 10−3, 3.19 × 10−3, and 1.49 × 10−2, respectively. n = 7, 9, 12, 6, 8, and 11 discs for the genotypes in (A), (B), (C), (D), (E), and (F), respectively. Scale bar, 50 µm. In each box and whisker plot, the box represents the interquartile, spanning from the first quartile to the third quartile, with a white line inside marking the median, and the whiskers show the range of the data, reaching the minimum and maximum values. Source data are provided as a Source Data file.
