Fig. 1: Intermittent fasting reduces aSyn-induced degeneration of dopaminergic neurons. | Nature Communications

Fig. 1: Intermittent fasting reduces aSyn-induced degeneration of dopaminergic neurons.

From: Intermittent fasting reduces alpha-synuclein pathology and functional decline in a mouse model of Parkinson’s disease

Fig. 1

A Experimental scheme. B Serum levels of BHB (mM). n = 6, two-way ANOVA, Tukey post-hoc test. ****: p < 0.0001. C Representative images of midbrain sections. Upper images: rAAV-aSyn–AL, lower images: rAAV-aSyn–IF. Overview: TH staining (gray). Scale bar: 500 µm. Insets: TH and human aSyn (hm aSyn) staining (gray), or both (TH: cyan, hm aSyn: magenta) of the indicated areas. Scale bar: 50 µm. D Representative images of striatal sections. Upper images: rAAV-aSyn–AL, lower images: rAAV-aSyn–IF. Overview: TH staining (gray) of both hemispheres. Scale bar: 500 µm. Insets: TH and human aSyn (hm aSyn) staining (gray), or both as overlay (TH: cyan, hm aSyn: magenta). Scale bar: 50 µm. E Normalized number of TH-positive neurons in the SN. Numbers were normalized to the non-injected hemisphere of the same animal. Box and whisker plots, middle line: median, +: mean, circles: individual animals; n = 7, two-way ANOVA, Tukey post-hoc test, p < 0.0001. TH neuron numbers are on Supplementary Fig. 1D. F Density of TH-positive fibers in the STR, normalized to the (i) contralateral hemisphere and (ii) to the rAAV-GFP–AL group. Box and whisker plots (see “methods”), middle line: median, +: mean, circles: individual animals; n = 7, two-way ANOVA, Tukey post-hoc test, p < 0,0001). Fiber densities are on Supplementary Fig. 1E. G Expression of GFP and hmSNCA in the SN. Box and whisker plots, middle line: median, +: mean, circles: individual animals; n = 5, two-way ANOVA, Tukey post-hoc test. H Normalized number of GFP- or human aSyn-positive neurons in the SN. Numbers were normalized to the (i) non-injected hemisphere of the same animal and (ii) AL. Box and whisker plots, middle line: median, +: mean, circles: individual animals; n = 7, two-way ANOVA, Tukey post-hoc test, p < 0.0001. I Density of GFP- or hmaSyn-positive fibers in the STR, normalized to the (i) contralateral hemisphere and (ii) to rAAV-GFP–AL. Box and whisker plots, middle line: median, +: mean, circles: individual animals; n = 7, two-way ANOVA, Tukey post-hoc test, p < 0.0001). Box and whiskers plots: box: 25th to 75th percentiles, whiskers: from the smallest to the largest value. Source data are provided as a Source Data file.

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