Fig. 10: Anti-inflammatory treatment ameliorates aSyn toxicity in vitro.

A Representative images of primary neuronal cultures transduced with rAAV-aSyn and treated as indicated on the top. Cultures were stained against MAP2, GFAP, human aSyn and p129S-aSyn. Scale bar: 20 µm. B LDH release in cultures treated as indicated and normalized to the positive (cultures treated with 1% Triton X-100) and negative (empty medium) controls, and for rAAV-GFP. Box and whisker plots, middle line: median, circles: independent experiments/preparations; n = 5, one-way ANOVA followed by Tukey post-hoc test. p-values are indicated on the graph. C Quantification of the area fraction positive for the MAP2-staining in rAAV-aSyn-transduced primary cultures, normalized to rAAV-GFP. Box and whisker plots, middle line: median, circles: independent experiments/preparations; n = 5, one-way ANOVA followed by Tukey post-hoc test. p-values are indicated on the graph. D Quantification of the area fraction positive GFAP, normalized for rAAV-GFP. Box and whisker plots, middle line: median, circles: independent experiments/preparations; n = 5, one-way ANOVA followed by Tukey post-hoc test. p-values are indicated on the graph. Data including the rAAV-GFP treated cultures is included as Supplement to Fig. 10. Box and whiskers plots: box: 25th to 75th percentiles, whiskers: from the smallest to the largest value. Source data are provided as a Source Data file.