Fig. 7: Intermittent fasting induces profound transcriptome changes in the ventral midbrain of mice injected with rAAV-aSyn. | Nature Communications

Fig. 7: Intermittent fasting induces profound transcriptome changes in the ventral midbrain of mice injected with rAAV-aSyn.

From: Intermittent fasting reduces alpha-synuclein pathology and functional decline in a mouse model of Parkinson’s disease

Fig. 7

Bulk RNA sequencing was performed from the ventral midbrain of mice transduced with rAAV-GFP or rAAV-aSyn, and subjected to diet control. A Heatmap shows differentially expressed genes between aSyn-AL and aSyn-IF groups. Z-scores of genes of individual mice are used to generate the heatmap, note: only every 15th gene is labeled with names. B Volcano plot shows genes detected in aSyn-AL and aSyn-IF. Blue: down-regulated DEGs, red: up-regulated DEGs. For the graph, log2 fold change (log2FC) and log10 p-values were used. Genes are considered as DEG, when the log2FC > 0.5 or log2FC < −0.5, and log10(p) > 1.3 (p < 0.05). Genes selected for further validation are highlighted in (A, B). two-sided t-test, p < 0.05. C Gene Ontology (GO) analysis of DEGs. Genes, selected as in (B), were significantly regulated both in aSyn-AL vs. GFP-AL and aSyn-AL vs. aSyn-IF. Fisher’s exact test was used to compute the p-values and enrichment factors for each ontology category. Source data are provided as a Source Data file.

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