Fig. 4: Intranasal vaccination with RBD-fused engineered HSA in Tg32-hFc mice induces robust antigen-specific antibodies beyond that of intramuscular vaccination and of an alternative carrier. | Nature Communications

Fig. 4: Intranasal vaccination with RBD-fused engineered HSA in Tg32-hFc mice induces robust antigen-specific antibodies beyond that of intramuscular vaccination and of an alternative carrier.

From: An intranasal subunit vaccine induces protective systemic and mucosal antibody immunity against respiratory viruses in mouse models

Fig. 4

a Illustration of Tg32-hFc mice expressing human FcRn and chimeric IgG1 with human Fc (chIgG1), and RBD-specific IgG and IgA in sera, BALF, nose and saliva of female mice at endpoint (prime dose: 6.2 μg RBD and 20.0 μg RBD-QMP in combination with 20 μg CpG), and the ability of the antibodies in sera and BALF to b inhibit human ACE2-RBD binding and c inhibit cellular infection by SARS-CoV-2 pseudovirus of the ancestral strain (Wuhan). d Percentage of antigen-specific B-cells in mediastinal lymph nodes. e Illustration of Tg32-hFc mice that were intranasally or intramuscularly vaccinated with RBD-QMP, and RBD-specific IgG and IgA in sera and BALF 5 weeks after initial vaccination of male mice (prime dose: 20.0 μg RBD-QMP in combination with 20 μg CpG) f RBD-specific IgG and IgA in sera, BALF, nose and saliva of female and male Tg32-hFc mice at endpoint (prime dose: 20.0 μg RBD-QMP (2 F, 3 M) and 22.0 μg RBD-Tf (3 F, 2 M) in combination with 20 μg CpG), and g the ability of the antibodies in sera and BALF to inhibit human ACE2-RBD binding. All serology was detected using FCBA. Bars indicating group mean ± SD with individual mice represented as a single datapoint (a RBD n = 5 and RBD-QMP n = 6, except nose RBD n = 3, b, e, f, g n = 5, except e RBD n = 3 (intranasal) or n = 4 (intramuscular)). Pseudovirus neutralization c presented as technical duplicates of pooled biological samples per group (RBD n = 5 and RBD-QMP n = 6). d All mediastinal lymph nodes per group (PBS n = 3, RBD and RBD-QMP n = 5) were merged and samples prepared in technical triplicate (PBS as duplicate) for flow cytometry. a, b, f, g Two-tailed unpaired t-test, and e Two-tailed Mann–Whitney test. ac, eg Partially created in BioRender86.

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