Fig. 2: Mice with BAT-specific Lamp2a knockdown show identical energy metabolism at TN.

a Immunoblotting for LAMP2A protein levels of BAT from male C57BL/6 mice 4 weeks after injection with AAV-shScramble or AAV-shLamp2a and totally housed at TN for 8 weeks. b–j Measurements of metabolic parameters of indicated mice housed at TN for 8 weeks, including body weight (b), n = 7 mice per group; fat mass (c), n = 7 mice per group; lean mass (d), n = 7 mice per group; BAT mass (e), n = 4 mice per group; oxygen consumption and quantitation of oxygen consumption (f), n = 4 mice per group; carbon dioxide production and quantitation of carbon dioxide production (g), n = 4 mice per group; EE and quantitation of EE (h), n = 4 mice per group; RER and quantitation of RER (i), n = 4 mice per group; GTT and AUC of GTT (j), n = 7 mice per group; ITT and AUC of ITT (k), n = 7 mice per group. l, m Histochemistry analysis, including representative hematoxylin-eosin staining of BAT (l), representative immunohistochemical UCP1 staining of BAT (m). Scale bar, 20 µm. n OCR analysis of BAT (n = 4 mice per group). o Immunoblotting for PGC1α and UCP1 protein levels of BAT lysate (representative of n = 3 independent experiments). p Quantification of o. Statistical significance was assessed by unpaired Student’s t-test. All results were expressed as means ± SEM, *p < 0.05, **p < 0.01, ***p < 0.005. Student’s two-tailed unpaired T-test for 2-group comparisons. Source data are provided as a Source Data file.