Fig. 4: Genome accessibility is limited in isolated MCF7 nuclei.
From: Nucleosome dynamics render heterochromatin accessible in living human cells
a Agarose gel electrophoresis of DpnI-digested genomic DNA purified from nuclei treated with increasing amounts of Dam. M, DNA marker. MboI, unmethylated control DNA fully digested at GATC sites by MboI. b Methylation of all the GATC sites in the human genome as a function of Dam concentration. Red line: methylation of the median GATC site; shading indicates the data range. c Active genes: Nucleosome phasing and methylation of the median GATC site in promoters or gene bodies as a function of Dam concentration. Red line and shading: median GATC site methylation with data range indicated. Grey profile: nucleosome dyad distribution in nuclei (MNase-seq data for MCF7 cells arbitrarily normalised to 30%). d The same analysis for inactive genes. e Possible ATP-dependent mechanisms for generating accessibility in living cells (based on the known activities of various ATP-dependent chromatin remodelers).
