Fig. 1: Genetic crosses between virulent HLJ and avirulent GD strains of Cryptosporidium parvum in vivo.
From: SKSR1 identified as key virulence factor in Cryptosporidium by genetic crossing
a Diagram of genetic crosses of HLJ and GD in mice and flow cytometric sorting of the progeny. b Image of oocysts harvested from the intestinal mucosa 4 days after coinfection of oocysts tagged with different fluorescent proteins and the sporozoites excysted from them. Scale bars = 2 µm. c Ratio of oocysts of each color in intestinal content of mice 4 days after coinfection, as determined by microscopy of samples (mean ± SD) from three independent experiments (the 2nd, 3rd, and 4th genetic crosses). d Confirmation of genetic recombination in single oocysts of progeny by read mapping of whole genome sequences. W587 and W595 represent the IDs of the sequenced single oocyst genomes. Two polymorphic sites relative to the parental sequences on the genomes in oocyst W595 are marked, indicating crossover of sequence types in this region. Source data are provided as a Source Data file.
