Fig. 3: HMGA1 negatively regulates STING expression. | Nature Communications

Fig. 3: HMGA1 negatively regulates STING expression.

From: High mobility group A1 (HMGA1) promotes esophageal squamous cell carcinoma progression by inhibiting STING-mediated anti-tumor immunity

Fig. 3

a Heatmap showing the expression of genes related to innate immunity in control and shHMGA1-30-1 cells from the RNA-seq analysis. b A cohort of 82 ESCC patients (70 males and 12 females) from the TCGA database was analyzed to evaluate the correlation between HMGA1 and STING expression. The data were sourced directly from aggregated TCGA database entries and used for analysis without modification. c, d Levels of STING in HMGA1-knockdown KYSE-30 cells (c) and HMGA1-overexpressing KYSE-70 cells (d) were analyzed by qPCR. e, f Expression of STING protein in HMGA1-knockdown KYSE-30 cells (e) and HMGA1-overexpressing KYSE-70 cells (f). g Levels of Hmga1 and Sting in ESCCs from Hmga1flox/flox and Hmga1flox/floxK14 mice analyzed by qPCR. h The samples derive from the same experiment, but different gels for HMGA1, STING, and another for GAPDH were processed in parallel. GAPDH served as a loading control. i Levels of Hmga1 and Sting in ESCCs from Hmga1KI/KI and Hmga1KI/KIK14 mice analyzed by qPCR. j The samples derive from the same experiment, but different gels for HMGA1, STING, and another for GAPDH were processed in parallel. k, l Expression and quantification of HMGA1 and STING in the esophagus of Hmga1flox/flox and Hmga1flox/floxK14 mice (k) and Hmga1KI/KI and Hmga1KI/KIK14 mice (l). Mice were treated with 4NQO to induce ESCC. At the end of the induction, murine esophagus tissues were isolated, fixed, and stained via IHC. Representative images are presented in the left panels, and quantifications of IHC staining are shown in the right panels of k and l. Scale bar: 20 μm. TCGA: The Cancer Genome Atlas. Data are presented as mean ± SD. Statistical significance was determined by two-tailed unpaired t-test (c, d, g, i, k, l), or Pearson correlation coefficient (b). Sample sizes: (c, d) n  =  4; (e, f) n  =  3 independent experiments; (g–j) n  =  3; (k, l) n  =  6 independent mice. Image in e, f, h, j, k, and l shows representative results from three independent experiments. Source data are provided in the Source Data file.

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