Fig. 4: Residues Glu255 and His307 in the p10 ___domain regulate the self-cleavage activation and substrate processing.
From: Structural determinants for pH-dependent activation of a plant metacaspase
a Interactions involving Glu255 and His307 at pH 4.2, 5.5, and 7.5. Interactions and water at each pH are displayed as each pH’s respective color, but with pH 7.5 displayed as red. b Comparison of representative frames of the pH 4.2 (gray), 5.5 (purple), and 7.5 (marine/orange) simulations showing interactions involving Glu255 and His307. c Self-cleavage in E255A. d Self-cleavage in H309A. e Substrate processing of GST-PROPEP1 by E255A. f Substrate processing of GST-PROPEP1 by H309A. WT: For the wild-type AtMC9 sample in (c, d), pH 8 was used in the incubation to compare with the mutant. Data are representative of two independent experiments for (c–f). Source data are provided as a Source Data file.
