Fig. 5: Ttyh1 prevents C1P-induced lipid droplet accumulation. | Nature Communications

Fig. 5: Ttyh1 prevents C1P-induced lipid droplet accumulation.

From: Endolysosomal processing of neuron-derived signaling lipids regulates autophagy and lipid droplet degradation in astrocytes

Fig. 5

a Loss of Ttyh1 sensitizes C1P-induced TAG accumulation in astrocytes. Cellular TAG contents in cKO astrocytes were measured after treatment with 0 (0.1% DMSO), 0.5, or 5 µM of C1P in 0.1% BSA-supplemented culture media for 2 h. Data were presented as mean ±  SEM. Number of independent lipid extracts (n) is shown at the bottom of each bar. One-way ANOVA (F = 581.7; df = 20) followed by Bonferroni’s multiple comparisons post hoc test. b, c Loss of Ttyh1 exacerbates C1P-induced LD buildup in astrocytes. Representative confocal images in (b) show cKO astrocytes stained with BODIPY (green) and DAPI (blue) after indicated treatments. Quantifications of BODIPY signals in (c) reveal LD contents in Ttyh1 WT and KO astrocytes. BODIPY-positive area per cell values were normalized to the mean values of Ttyh1 WT control. Violin plots show median (solid line), interquartile range (dotted line), and probability density of data (smoothed shape). Number of astrocytes (n) from ≥3 independent experiments is shown underneath each violin plot. Mann–Whitney test. Source data are provided as a Source Data file.

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