Fig. 3: Cryo-EM structure of the human TREX-2^M/DDX39B complex with ADP.

a Schematics for generating the photo-crosslinked TREX-2^M/DDX39B complex and Coomassie-stained SDS-PAGE of the cryo-EM sample. This experiment has been repeated three times independently with similar results. b Cryo-EM map of the TREX-2^M/DDX39B complex determined at 3.25 Å resolution (EMD-46981), low-pass filtered at 6 Å resolution. The GANP, PCID2, and DSS1 subunits of TREX-2^M are colored in green, yellow, and blue, respectively. The NTM, RecA1, and RecA2 domains of DDX39B are colored in red, pink, and purple, respectively. c Cryo-EM map of the TREX-2^M/DDX39B^NTM+RecA1 complex determined at 2.79 Å resolution (EMD-46982). d Structure of the TREX-2^M/DDX39B^NTM+RecA1 complex (PDB 9DLP). DDX39B is observed in a post-hydrolysis state bound to ADP. e–g TREX-2^M interfaces with NTM-N (e), NTM-C (f), and RecA1 (g, arrowhead indicates the trigger loop) of DDX39B. h Schematics of the interactions between TREX-2^M and DDX39B^NTM+RecA1. Source Data are provided as a Source Data file.