Fig. 8: Medial prefrontal, hippocampal, and amygdalar synaptic circuits through AMd.medial tip and AMv.
From: Distinct subnetworks of the mouse anterior thalamic nuclei
a Injection strategy used in MORF3 mice to validate the SUBv→AMd.medial tip→BLA disynaptic circuit. b A synaptophysin tagged anterograde AAV injection made in the SUBv labels terminals in the AMd.medial tip, while an AAVretro-Cre injection in the BLA back labels neurons in the AMd.medial tip. c 60x magnification of a BLA projecting AMd.medial tip neuron. d The same AMd.medial tip→BLA neuron in (c) merged with labeled synaptic terminals from the SUBv. e Note the close apposition of the neuron processes and terminals suggesting putative synaptic contacts of SUBv terminals onto the BLA projecting AMd.medial tip neuron. f, g Putative contacts were reconstructed and validated via Neurolucida (see “Methods” for details). h Injection strategy used in MORF3 mice to validate the SUBv→AMd.medial tip→PL disynaptic circuit. i A synaptophysin-tagged anterograde AAV injection made in the SUBv labels terminals in the AMd.medial tip, while an AAVretro-Cre injection in the PL back labels neurons in the AMd.medial tip. j 60x magnification of a PL projecting AMd.medial tip neuron. k The same AMd.medial tip→PL neuron in (j) merged with labeled synaptic terminals from the SUBv. l Close apposition of neuron processes and terminals suggests putative synaptic contacts of SUBv terminals onto the PL projecting AMd.medial tip neuron. m Putative contacts were validated via Neurolucida same as in (f–g). n The same injection strategy in a and h was applied in MORF3 mice to validate the ILA→AMv→PL circuit. o Synaptic contacts of ILA projections onto PL projecting AMv neurons were reconstructed and validated. See Supplementary Table 2 for structure abbreviations.
