Fig. 8: Model of cpzA3H-Vif-CUL5 E3 ligase complex. | Nature Communications

Fig. 8: Model of cpzA3H-Vif-CUL5 E3 ligase complex.

From: HIV-1 vif mediates ubiquitination of the proximal protomer in the APOBEC3H dimer to induce degradation

Fig. 8

Model based on the cryo-EM structure from this study and components from PDB IDs 7B5L, 7B5M, 7ONI, 8FVJ21,40,41. a cpzA3H-VCBC dimer structure (this study) is modeled with the CUL5 E3 ligase machinery, with proteins color-coded as follows: cpzA3H in green, Vif in cyan, CBFβ in dark gray, EloBC in light gray, members of CUL5 E3 ligase complex in gray, ARIH in beige, and ubiquitin in dark red. Gly76 of ubiquitin and Lys50 and Lys51 of cpzA3H are shown as spheres. This model depicts transition state 2 that is about to transfer ubiquitin to the target protein. b Distances between the cpzA3H lysine side chains and Gly76 of the E3-bound ubiquitin. Ubiquitination of these lysines, mediated by the Vif-CUL5 E3 ligase machinery, was detected by mass spectrometry.

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