Extended Data Fig. 9: Tailing status of pre-miRNAs and miRNAs in WT (Col-0) and hen1-8.
From: Prevalent cytidylation and uridylation of precursor miRNAs in Arabidopsis
a, b, Analysis of uridylation (a) and cytidylation (b) of Class I pre-miRNAs and miRNAs in WT (Col-0) and hen1-8. Each dot represents a pre-miRNA. The black and gray lines represent the median and the quartiles, respectively. Values are Class I pre-miRNAs from one biological repeat (n = 24). Percentage of uridylation or cytidylation was calculated to the total reads mapped to each pre-miRNA or miRNA. Mono: mono-uridylation or mono-cytidylation; All: total uridylation or cytidylation. c, d, Analysis of uridylation (c) and cytidylation (d) of individual Class I pre-miRNAs and miRNAs (miRNA locates in the 3 P strand) in WT (Col-0) and hen1-8. Error bars are s.e.m., n = 3. e, f, Percentage of 1-nt and 2-nt tails at position 1 (e) and 2 (f) of pre-miR158a and miR158a in WT (Col-0) and hen1–8. Percentage was calculated based on total tailed pre-miR158a and miR158a at position 1 (e) and 2 (f). The inset shows all cytidine-containing tails. Error bars are s.e.m., n = 3. Differences between WT (Col-0) and hen1–8 were evaluated by student t-tests (unpaired, two-sided). *P < 0.05.
