Extended Data Fig. 5: Loss of function of PDK1.1 and PDK1.2 impaired auxin distribution.
From: The lipid code-dependent phosphoswitch PDK1–D6PK activates PIN-mediated auxin efflux in Arabidopsis
Observation of the auxin responsive reporter DR5rev::GFP by CLSM indicated a dramatic decrease of the auxin maxima in pdk1.1 pdk1.2 (e–h) compared with Col-0 (a–d). Fused cotyledon exhibiting two sites of auxin maxima in light-grown 7-day-old seedlings of pdk1.1 pdk1.2 (e) compared with one of Col-0 (a); roots of light-grown 10-day-old seedlings (b and f); apical hooks of 10 µM ACC treated 4-day-old etiolated seedlings (c and g); roots of 10 µM ACC treated 4-day-old etiolated seedlings (d and h). Scale bars, 200 µm. n = 3 biologically independent experiments, with similar results obtained.
