Extended Data Fig. 6: Deficiency of PDK1.1 and PDK1.2 did not affect the polarity of PIN proteins.
From: The lipid code-dependent phosphoswitch PDK1–D6PK activates PIN-mediated auxin efflux in Arabidopsis
a, Deficiency of PDK1.1 and PDK1.2 did not change the polarity of PIN1-YFP. Four-day-old seedlings of pPIN1::PIN1-YFP in Col-0 and pPIN1::PIN1-YFP in pdk1.1 pdk1.2 were imaged by CLSM. Scale bars, 20 µm. n = 2 biologically independent experiments, with similar results obtained (a-g). b, Deficiency of PDK1.1 and PDK1.2 did not change the polarity of PIN2-GFP. Four-day-old seedlings of pPIN2::PIN2-GFP in Col-0 and pPIN2::PIN2-GFP in pdk1.1 pdk1.2 were imaged by CLSM. Scale bars, 20 µm. c–f, Deficiency of PDK1.1 and PDK1.2 did not change the polarity of PIN3-GFP. Four-day-old seedlings of pPIN3::PIN3-GFP in Col-0 and pPIN3::PIN3-GFP in pdk1.1 pdk1.2 were imaged by CLSM. (c) subcellular localisation of PIN3-GFP; (d) an amplified view of PIN3-GFP in the root stele; (e) a close view of PIN3-GFP in root columella cells; (f) a 3D-projection of PIN3-GFP localisation in root columella cells. The “Green Fire Blue” LUT was used for photo visualization based on fluorescence intensity by Fiji. Scale bars, 20 µm. g, A transverse view of the root columella cells revealed the overproliferation, with an enlarged region expressing PIN3-GFP. Four-day-old seedlings of pPIN3::PIN3-GFP in Col-0 and pPIN3::PIN3-GFP in pdk1.1 pdk1.2 were stained with PI, and imaged by CLSM, at the position as marked in the left image. Scale bars, 20 µm.
