Extended Data Fig. 8: Functional pPDK1.1::Venus-PDK1.1 localised at both cytoplasm and the basal side of PM.

a, pPDK1.1::Venus-PDK1.1 rescued the growth defects of pdk1.1 pdk1.2. 25-day-old adult plants were observed and representative photos are shown. Scale bar, 2 cm. b, pPDK1.1::Venus-PDK1.1 rescued the lateral root defects of pdk1.1 pdk1.2. 10-day-old seedlings were observed and representative photos are shown. Scale bar, 2 cm. c, pPDK1.1::Venus-PDK1.1 rescued the defects of pdk1.1 pdk1.2 in the hypocotyl gravitropic response. 4-day-old etiolated seedlings were observed and representative photos are shown. Scale bar, 2 cm. d, Venus-fused PDK1.1 localised to both cytoplasm and PM, especially with a predominant presence at the basal side of PM. Four-day-old seedlings of pPDK1.1::Venus-PDK1.1 were imaged by CLSM. Open arrowheads indicate the basal polar localisation. The “Green Fire Blue” LUT was used for photo visualization based on fluorescence intensity by Fiji. Scale bar, 20 µm. e, Venus-PDK1.1 localized to cytoplasm (upper image) in interphase tobacco BY-2 cells, and the association with PM was obvious only during cytokinesis (arrow in lower image). An XVE>>Venus-PDK1.1 line was induced for 48 h with 1 μM β-estradiol and were then imaged by spinning disk confocal microscope. Scale bar, 10 μm. n = 3, 3, 2, 3, and 2 biologically independent experiments for (a), (b), (c), (d), and (e) respectively, with similar results obtained.