Extended Data Fig. 6: Role of the conserved stem region on oligomerization and activity of GalS1.
From: Structural and biochemical insight into a modular β-1,4-galactan synthase in plants
Stem region interaction across monomers in dimeric GalS1. b, Dim plot 2D interaction map of residues that interact between stem regions in GalS1 homodimer. c, Comparison of GalT activity and d, AraT activity in WT and GalS1ΔSTEM variant. The values shown are mean values (bar) ± standard deviation (error bars) of a representative experiment for n = 3 technical replicates (red circles) and plotted using GraphPad Prism 9.5.0. e, Change in oligomerization state of GalS1ΔSTEM as compared to GalS1WT determined by SEC-MALS. f, Web logo showing conservation of residues beyond residue 96 in the predicted stem domains of plant GT92 proteins from Salix suchowensis, Populus alba, Populus trichocarpa, Jatropha curcas, Hevea brasiliensis, Ricinus communis, Manihot esculenta, Olea europaea, Carica papaya, Herrania umbratica, Cephalotus follicularis, Eucalyptus grandis, Cucurbita moschata, Camellia sinensis, Mangifera indica, Punica granatum, Pistacia vera, Durio zibethinus, Telopea speciosissima, Vitis riparia, Gossypium hirsutum, Quercus lobata, Quercus suber, Thalictrum thalictroides, Syzygium oleosum, Sesamum indicum, Gossypium australe, Morus notabilis, and Vitis vinifera. The sequence number on the X-axis refers to native GalS1 from Populus trichocarpa and Y-axis is in bits with error bars that indicate an approximate Bayesian 95% confidence interval. Thirty-three (n = 33) species of plants were examined in this experiment.
