Extended Data Fig. 1: PAGA-dependent regulation of gene expression as determined by RNA-seq.

a, Scatter plots showing the correlation of gene expression changes between the spc mutant and the other PAGA mutants or the ada2b mutant. P values were generated by two-sided Pearson correlation test. b, Venn diagrams showing the overlap of down- and up-regulated DEGs (log2 FC > 1 or < −1, FDR < 0.05) in spc, spc ing1, and ada2a spc. P values were determined by the hypergeometric test (one-tailed). c, Heatmap showing the correlation of the effects of PAGA mutants on gene expression. Values indicate correlation coefficients.