Extended Data Fig. 5: Target gene silencing by HDA6-ZF, MSI1-ZF, and histone deacetylation.
From: A gene silencing screen uncovers diverse tools for targeted gene repression in Arabidopsis
a, Screenshots indicating H3K9ac, H3K14ac and H3 ChIP–seq signals over two representative ZF off-target sites in fwa and T2 transgenic lines of HDA6-ZF and MSI1-ZF. b-c, Heat maps and metaplots showing normalized H3K9ac (b) and H3K14ac (c) ChIP–seq signals over three clusters of ZF off-target sites in HDA6-ZF and MSI1-ZF versus fwa (left panel). H3K9ac (b) and H3K14ac (c) cluster 1, 2, 3 represents high, medium, and low levels of preexisting ChIP–seq signals of H3K9ac (b) and H3K14ac (c), respectively (right panel).
