Fig. 2: The inheritability of target gene silencing in the T2 lines of ZF fusions.
From: A gene silencing screen uncovers diverse tools for targeted gene repression in Arabidopsis
a, CG, CHG and CHH DNA methylation levels over FWA promoter regions in fwa, Col-0, ZF-SUVH2 transgenic T2 line (+) and ZF-SUVH2 segregant T2 line (−), measured by BS-PCR-seq. Pink vertical boxes indicate ZF binding sites. b, Flowering time of ZF fusions T2 segregant lines (−) and transgenic lines (+). The number of plants with 20 or fewer leaves are indicated. c, Western blot showing the protein expression levels of ZF fusion T2 transgenic lines with early flowering phenotype (left three samples) and late flowering phenotype (right three samples). The top panels of each ZF fusion were blotted with anti-FLAG, and the bottom panels were stained by Ponceau using as loading controls; each experiment was repeated twice independently with similar results.
