Fig. 5: Targeted gene silencing by ELF7, CPL2 and Pol II transcription disruption.
From: A gene silencing screen uncovers diverse tools for targeted gene repression in Arabidopsis
a, Screenshots of Pol II Ser5 and H3 ChIP–seq signals over FWA region in fwa, ELF7-ZF and HD2A-ZF. b, Heat maps and metaplots representing normalized Pol II Ser5 ChIP–seq signals in the representative T2 lines of ELF7-ZF and HD2A-ZF versus fwa, over ZF off-target sites and the shuffled sites (n = 6,091), respectively. c, Heat map and metaplot showing normalized Pol II Ser5 ChIP–seq signals over ELF7-FLAG ChIP–seq peaks (n = 16,768) and ELF7 peaks shuffle in elf7-3 versus Col-0. d, Screenshots of Pol II Ser5 ChIP–seq signals over FWA in fwa and a CPL2-ZF representative T2 transgenic line. e, Heat map and metaplot depicting the normalized Pol II Ser5 ChIP–seq signals over ZF off-target sites that had pre-existing Pol II Ser5 ChIP–seq signals (n = 2,000) and the shuffled sites in CPL2-ZF versus fwa.
