Fig. 6: Predicting the efficacy of different effector proteins by using machine learning models and some effector protein also worked in SunTag system.
From: A gene silencing screen uncovers diverse tools for targeted gene repression in Arabidopsis
a, Screenshots displaying RNA-seq levels over a representative ZF off-target gene AT3G13470 in fwa-1, HD2A-ZF, HD2B-ZF, HD2C-ZF, ELF7-ZF, fwa-2, LHP1-ZF and CPL2-ZF. FLAG-ZF ChIP–seq indicates the binding site of ZF. b, Bar chart indicating the accuracy of ten-fold cross-validation in each ZF line. c, Dot plots displaying leaf numbers of Col-0, fwa rdr6 and representative SunTag T2 lines. d, qRT–PCR analysis showing the relative mRNA level of FWA in Col-0, fwa rdr6 and representative SunTag T2 lines. Error bars indicate the standard error of the mean (s.e.m.) of three technical replicates of each sample; data are presented as mean ± s.e.m.
