Extended Data Fig. 3: Target gene silencing by H3K4me3 demethylation.
From: A gene silencing screen uncovers diverse tools for targeted gene repression in Arabidopsis
a, Screenshots of H3K4me3 and H3 ChIP–seq signals over a representative ZF off-target site in fwa and T2 transgenic lines of JMJ14-ZF and ELF7-ZF. The FLAG-ZF ChIP–seq signals indicate ZF binding sites. b, Heat maps and metaplots showing the normalized H3K4me3 ChIP–seq signals over three clusters of ZF off-target sites in JMJ14-ZF and ELF7-ZF transgenic lines versus fwa (left panel). H3K4me3-cluster 1, 2, 3 represent high, medium, and low levels of preexisting H3K4me3 ChIP–seq signals, respectively (right panel). c, Screenshots of H3K27me3 and H3 ChIP–seq signals in fwa and a representative T2 line of JMJ14-ZF over FWA. d, Heat maps and metaplot showing the normalized H3K27me3 ChIP–seq signals over ZF off-target sites and shuffled sites (n = 6,091) in JMJ14-ZF T2 transgenic line versus fwa.
