Fig. 1: PLAD chromatin detaches from the nuclear periphery under heat stress.
From: The plant nuclear lamina disassembles to regulate genome folding in stress conditions
a, The experiment setup. b,c, Representative FISH images (b) and quantification (c) showing the localization of PLAD (green) and non-PLAD (red) chromatin with respect to the nuclear periphery. The box plots in c indicate the median (line within the box), the lower and upper quartiles (box), margined by the largest and smallest data points that are still within the interval of 1.5 times the interquartile range from the box (whiskers); outliers are not shown. For chromosome 1, n = 53 for mock and n = 50 for heat-stress treatment. For chromosome 3, n = 52 for mock and n = 48 for heat stress. The p values shown above the box plots were obtained from two-sided Mann–Whitney U-tests. M and H stand for mock and heat-stress treatment, respectively. d, Representative chromosome painting images showing altered localization patterns of a ~1.3 Mb genomic region after heat treatment. This probed genomic region mainly belongs to PLADs. The sketch shown above depicts the ___location of the probed region. e, ChIP–qPCR showing the interactions between CRWN1 and two PLAD loci, which overlap with gene loci AT1G65750 (PLAD1) and AT3G29767 (PLAD2), respectively. ‘Ctrl’ refers to a non-PLAD locus. The relative fold enrichment was calculated by using the TUB2 genomic locus as the reference. Error bars mean standard deviation of three biological replicates; p values indicate results of two-sided t-tests. Images in b and d are representatives from two independent experiments with similar patterns.
