Extended Data Fig. 8: Change of thrum to long homostyle flowers caused by loss-of-function mutation in S-ELF3. | Nature Plants

Extended Data Fig. 8: Change of thrum to long homostyle flowers caused by loss-of-function mutation in S-ELF3.

From: Genome sequencing reveals the genetic architecture of heterostyly and domestication history of common buckwheat

Extended Data Fig. 8

a, Illustration of phenotype changes by loss-of-function mutation in S-ELF3 of the EMS mutant. b, Pollen tube growth after three hours of crossing the F. esculentum EMS mutant (long homostyle) pistil with the wild type thrum pollen (left panel) and the wild type pin pollen (right panel). Thrum pollen tubes reached the ovule (left panel, yellow arrowhead), whereas pin pollen tubes were arrested in the long styles of the EMS mutant (right panel, red arrowheads). Scale bar = 0.2mm. c, Style length of 10 wild type thrum, wild type pin, F. esculentum PL4 (long homostyle), and F. esculentum EMS mutant (long homostyle) flowers. d, Anther filament length of 10 wild type thrum, wild type pin, F. esculentum PL4 (long homostyle), and F. esculentum EMS mutant (long homostyle) flowers. The box plots show the median and first and third quartiles with the whiskers extending to 1.5 times the inter-quartile range. All wild types used here are F. esculentum cv. Harunoibuki.

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