Extended Data Fig. 1: Effect of cold storage duration on lily sprouting and flower differentiation.
From: Epigenetic silencing of callose synthase by VIL1 promotes bud-growth transition in lily bulbs
a, Bulb growth transition: cold storage (4 °C) accelerates bulb growth transition, while frozen storage (-1.5 °C) delays it for long-term storage. The distance between the bud and the bulb tip shows the bud elongation during storage. Data are presented as mean ± s.d. of three biological replicates (n = 5 bulbs per replicate). b, Bulb sprouting: effect of cold storage (4 °C) duration on bulb sprouting. Data are presented as mean ± s.d. of three biological replicates (n = 100 bulbs per sample; two-sided Student’s t-test). c, Plant height: effect of cold storage (4 °C) duration on plant height 90 days after planting. Data are presented as mean ± s.d. of three biological replicates (n = 100 bulbs per sample; two-sided Student’s t-test; ns: not significant and p > 0.05). d, Central bud development: central bud development at different stages using scanning electron microscopy. In DBs, the central bud was flat on the basal plate. In GTBs, the central bud grew up to2/3 bulb height and the SAM was evident. After sprouting, the SAM of the central bud was converted into FAM. The highlighted area in red indicates the tepal primordium. Consistent results were obtained in at least three independent bulbs. DB: dormant bulbs (cold storage at 4 °C for 0 weeks); GTB: growth transited bulbs (cold storage at 4 °C for 8 weeks); FAM: flower apical meristem; SAM: shoot apical meristem; SB: sprouting bulbs (cold storage at 4 °C for 8 weeks and then sprouting at 12 °C for 2 weeks); TP: Tepal primordium. Scale bar, 2 mm.
