Extended Data Fig. 3: CaM7 binds to GLR3.3 CTD in a Ca2+-dose dependent manner.
a MBP–C2 was immobilized with amylose beads to pull down GST–CaM7 in the presence of Ca2+ concentration gradients or 2 mM EGTA. Three independent replicates were performed with similar results. b Control titration showing no obvious heat changes when the buffer without CBD peptide was titrated into 6His–CaM7 protein. Related to Fig. 2.
