Extended Data Fig. 5: Sample maps of AZG1 structures.
From: Structures and mechanisms of the Arabidopsis cytokinin transporter AZG1
a-g, Sample maps at 14 transmembrane helices (TMs) of (a) AZG1apo/pH7.4, (b) AZG1adenine/pH5.5, (c) AZG1T440Y/apo/pH7.4, (d) AZG1tZ/pH7.4, (e) AZG1tZ/pH5.5, (f) AZG1BAP/pH7.4, and (g) AZG1kinetin/pH7.4. The density is shown as a mask around each TM at the level of 0.02 in UCSF ChimeraX. h-n, Density around the substrate-binding site in (h) AZG1apo/pH7.4, (i) AZG1adenine/pH5.5, (j) AZG1T440Y/apo/pH7.4, (k) AZG1tZ/pH7.4, (l) AZG1tZ/pH5.5, (m) AZG1BAP/pH7.4, and (n) AZG1kinetin/pH7.4 at the contour level of around 6 σ in Coot. The dashed circle marks no density of substrate in the map of AZG1T440Y/apo/pH7.4.
