Fig. 1: Pre-mRNA splicing events in WT and grp20.

a, Relative expression levels of GRP20 in several Arabidopsis organs and structures: stage 1–5 flowers (including the inflorescence meristem), stage 6–11 flowers, stage 12–14 flowers, stems, roots, siliques, rosette leaves and cauline leaves. GRP20 is generally expressed in multiple tissues. The data are presented as mean ± s.e.m. from three biological replicates. b, The GRP20 protein ___domain structure, with an NLS, an RBD and an HDR. c, A Venn diagram of overlap for genes with splicing defects in flowers and/or leaves. d, GO category analysis of genes with defective transcripts in flower-specific, flower and leaf overlapping, and leaf-specific groups. The GO categories are shown for those with FDR < 0.05 (Mann–Whitney U test with 95% confidence intervals). GO enrichment is ranked 1–9 from yellow to red. Red indicates higher enrichment. ^aCategories highly enriched in the flower: histone and DNA modification enzymes, transcription factors for flower development, cell-division-related genes, ABCDE floral homeotic genes (MADS-box and AP2 family genes), LOB ___domain genes and lipid synthesis. ^bCategories highly enriched in the leaf: genes for signal transduction and for drought and immune stress response. ^cCategories highly enriched in both the leaf and flower: transcription factors for leaf development, DNA replication, RNA splicing, flowering time, ground tissue development, starch synthesis, lipid binding proteins and protein ubiquitination and those that broadly respond to temperature, hormone, osmotic stress, salt stress and lipids. TF, transcription factor.