Fig. 6: RBD is required for flower development.
a, The flower phenotypes of grp20-1 pi-1, grp20-1 ag-1 and grp20-1 as2-1 double mutants. Scale bar = 1 mm. The yellow arrows indicate the stronger phenotypes in double mutants compared with single mutants: more stigmatic tissues in grp20-1 pi-1 and shorter petals in grp20-1 as2-1. The asterisk indicates an abnormal chimeric organ. The yellow letters and numbers at the top right of each panel indicate corresponding organs and numbers in the flower. P, petal; S, stamen; St, stigmatic tissue. b, Number of floral organs in flowers of single and double mutants. The number of sepal-like organs in the second whorl and stigmatic tissues in pi-1 and grp20-1 pi-1 (flower counts: pi-1, 19 for sepal-like organs and 14 for stigmatic tissues; grp20-1 pi-1: 19 for sepal-like organs and 19 for stigmatic tissues). The number of first-whorl sepals in ag-1 and grp20-1 ag-1 (flower counts: ag-1, 19; grp20-1 ag-1, 19). The number of stamens in as2-1 and grp20-1 as2-1 (flower counts: as2-1, 20; grp20-1 as2-1, 21). From left to right, P = 0.041, 5.24 × 10−6, 0.015 and 8.74 × 10−4. c–e, Analyses of transgenic plants expressing GRP20 proteins with normal or mutant RBD (RBDm), as shown in Extended Data Fig. 9a. c, Relative protein expression levels in transgenic plants. Anti-histone 3 was used as a loading control. d, Mature flowers in transgenic plants. Yellow letters at the top right of each panel indicate the organ and corresponding number. Scale bars = 1 mm. e, Floral organ numbers in transgenic plants. Flower counts: ProGRP20::GRP20-YFP grp20, 25; ProGRP20::GRP20 (RBDm)-YFP grp20, 25; and grp20-1, 25. From left to right, for ‘Sepal’, P = 0.042 and 0.043; for ‘Petal’, P = 0.0024 and 2.08 × 10−5; for ‘Stamen’, P = 0.0085 and 0.0022; for ‘Carpel’, P = 0.033 and 0.027. For b and e, data are presented as mean ± s.d. for indicated flower counts; *P < 0.05; **P < 0.01, two-sided Student’s t-test.
