Extended Data Fig. 10: DCL1, SE and HYL1 impact RSS of pri-miRNAs.
From: Parallel degradome-seq and DMS-MaPseq substantially revise the miRNA biogenesis atlas in Arabidopsis
(a) Gini index of 67 common pri-miRNAs in Col-0, dcl1-9, hyl1-2 and se-1. P value by Wilcoxon test. The data are from three biological replicates for Col-0, dcl1-9 and hyl1-2, but two biological replicates for se-1. P (dcl1-9 vs Col-0) = 0.43, P (hyl1-2 vs Col-0) = 0.073, P (se-1 vs Col-0) = 0.0015. P value by Wilcoxon test. Centres of the boxes represent the median values. Upper bound and lower bound show the first and the third quartiles respectively. Whiskers indicate data within 1.5× the interquartile range of both quartiles. (b-d) Examples of SBTL-processed pri-miR447b (b), SLTB-processed pri-miR319a (c) and bidirectional-processed pri-miR166a (d) that show structural difference of pri-miRNAs in dcl1-9, hyl1-2 and se-1 compared to Col-0. Dotted boxes indicated structural differences in mutants. (e) Re-design of a known amiR backbone from pri-miR159a. An existing amiR backbone of pri-miR159a (top panel). Re-designing of the amiR backbone of pri-miR159a (bottom panel). amiR sequence is labelled with purple.
