Extended Data Fig. 3: Dexamethasone itself does not affect the expression of QC markers.

a: pWOX5»HAN expression in Col-0 QC184 after DEX induction. Confocal images showing erDsRed expression in mock-treated roots and after germination on 5 µM DEX for six days (a). Yellow arrowheads indicate QC position. b: Col-0 QC184 roots are unaffected by the induction of pWOX5»HAN. c-d: QC184 expression is not affected by germination on 5 µM DEX-containing medium for six days in the indicated genotypes. e-f: pWOX5:NLS-GUS (e) and QC25 (f) expression is not affected by germination on 5 µM DEX-containing medium. GUS signals (b-f) are shown in blue, and starch granules after Lugol staining in purple. In a-f, The images shown are representative of n > 20 independent roots that were measured and produced similar results. Scale bars: 20 μm.