Extended Data Fig. 3: HvWAKL20 interacts with and phosphorylate the NB-ARC ___domain of BSR1. | Nature Plants

Extended Data Fig. 3: HvWAKL20 interacts with and phosphorylate the NB-ARC ___domain of BSR1.

From: A cell wall-associated kinase phosphorylates NLR immune receptor to negatively regulate resistosome formation

Extended Data Fig. 3

a, BiFC assay to determine the interactions between HvWAKL20 and BSR1 NB-ARC ___domain. YFPc-fused bsr1, a null allele of BSR1, served as a negative control. Scale bars, 20 μm. b, Immunoblot analysis to validate protein expression shown in (a). c, MBP pull-down assay to test the interaction between the HvWAKL20 kinase ___domain (KD) or its variant and BSR1 NB-ARC ___domain. d, e, HvWAKL20 phosphorylates the BSR1 NB-LRR (d) and NB-ARC (e) domains in vitro. NB-ARC and NB-LRR domains were incubated with purified HvWAKL20 kinase ___domain respectively. The kinase-dead mutant HvWAKL20K387E served as the negative control.

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