Extended Data Fig. 4: MPK6 and SnRK2.6 cannot phosphorylate the TIR ___domain of SNC1.
From: TIR immune signalling is blocked by phosphorylation to maintain plant growth
a,b, Phosphorylation of wild-type SNC1TIR by CPK3, MPK6 or SnRK2.6. GST-CPK3 was used as a positive control. Autoradiography (top) and Coomassie staining (bottom) exhibit phosphorylation and the loading of recombinant MBP-MAPK6, MKK4DD, GST-CPK3, MBP-SnRK2.6 and GST-SNC1TIR (a). The anti-thiophosphate ester antibody was used to detect the phosphorylation of SNC1TIR, CPK3, MAPK6 and SnRK2.6 (b, top panel). The anti-MBP and anti-GST antibodies were used to detect the loading of recombinant GST-SNC1TIR, MBP-MAPK6, MBP-MKK4DD, and MBP-SnRK2.6 (b, middle panel and bottom panel). All experiments were repeated at least three times independently with similar results.
