Fig. 1: Temporal diagram design for physiological and metabolic experiments in rats. | npj Aging

Fig. 1: Temporal diagram design for physiological and metabolic experiments in rats.

From: Oral supplementation of nicotinamide riboside alters intestinal microbial composition in rats and mice, but not humans

Fig. 1

Rats were divided into four groups (n = 8) based on body weight upon arrival and received a standard chow diet and water. During the 12-week study period, they were fed an experimental 60% high-fat diet (HFD) or a matched control diet (CD) with 10% fat ad libitum in combination with 300 mg/kg/day nicotinamide riboside (NR) or vehicle (water, VE). During this period, they were subjected to an oral glucose tolerance test (oGTT, week 6) and an insulin tolerance test (ITT, week 7). In addition, fecal samples were collected at 5 time points, intestinal content from distinct sites was taken for 16S rRNA gene sequencing, body weight gain was monitored weekly, body composition was measured every second week and gas exchange, as well as feed intake, was assessed using metabolic chambers.

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