Fig. 3: Hepatic NAD+-related metabolites, cholesterol measurements, histological grading, triglycerides and tryptophan quantification in rats. | npj Aging

Fig. 3: Hepatic NAD+-related metabolites, cholesterol measurements, histological grading, triglycerides and tryptophan quantification in rats.

From: Oral supplementation of nicotinamide riboside alters intestinal microbial composition in rats and mice, but not humans

Fig. 3

A Hepatic levels of NAD+, NAMN, NAAD, Me2PY/Me4PY and ADPR. Diet effect: #p < 0.05, NR effect: *p < 0.05, **p < 0.01, ***p < 0.001. B Plasma levels of HDL and LDL/VLDL. NR effect: *p < 0.05. C Lipid score (0–4) shown as median and individual values. Diet effect: ###p < 0.001. D Hepatic triglycerides quantification. Diet effect: ##p < 0.01. E Steatosis score (0–3) shown as median and individual values. F Representative hematoxylin and eosin (H&E) staining used to assess steatosis score. Scale bar: 100 µm. G Representative Oil Red staining used to assess lipid score. Scale bar: 100 µm. H Hepatic and Plasma levels of tryptophan (TRP). NR effect: **p < 0.01. Data are shown as mean ± SEM if not otherwise specified. n = 6–8. CD control diet, HFD high-fat diet, NR nicotinamide riboside, VE vehicle.

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